A rapid and accurate method for the stem cell viability evaluation. The case of the thawed umbilical cord blood

Vassilios Katsares, Anastasia Petsa, Antigonos Felesakis, Zissis Paparidis, Eleni Nikolaidou, Sofia Gargani, Iliana Karvounidou, Karina-Alina Ardelean, Nikolaos Grigoriadis and John Grigoriadis

Biogenea-Cellgenea Ltd, Trade Centre "Plateia", Tsimiski 43, GR-54623, Thessaloniki, Greece

Correspondence to:

Vassilios Katsares, Ph.D., Biogenea-Cellgenea Ltd, Trade Centre "Plateia",

Tsimiski 43, GR-54623, Thessaloniki, Greece

Tel.: +302310282835, Fax: +302310282867, e-mail: Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από κακόβουλη χρήση. Χρειάζεται να ενεργοποιήσετε την Javascript για να τη δείτε.

Abstract:

The umbilical cord blood is a major source of hematopoietic progenitor cells. These cells can be cryopreserved for possible future use. In order to evaluate the viability of cryopreserved progenitor cells, a small amount of cells is thawed and subjected to the trypan blue exclusion test in order to count the dead (blue) cells and the live cells (white).

We used the Promega Glo-Max luminometer along with the CytoTox-Glo Cytotoxicity kit and the manufacturer's protocol as an alternative and rapid assay compared to the traditional trypan blue exclusion test.

The calculated viability percentages were similar between the two assays, but the counting time for a whole 96-well plate is only 30 min.

The CytoTox-Glo kit is a time-saving assay for the accurate evaluation of progenitor cell viability following cryopreservation and thawing.

Keywords: